ABSTRACT
Objective To observe the peroxidase body growth activated receptor γ (PPARγ) agonist rosiglitazone on acute pancreatitis in mice with hepatic injury and to investigate the mechanism of hepatic injury .Methods Seventy‐two male Kunming mice were randomly allocated into three groups(24 cases for each group):acute pancreatitis group(AP group) ,rosiglitazone group (AP‐ROS group) ,saline group(NS group) .Mice were killed at 6 ,12 and 24 h after induction of acute pancreatitis .Serum amylase , ALT and AST activities were measured .The expressions of NF‐κB and PPARγmRNA were assessed by RT‐PCR .The expressions of NF‐κB and PPARγ protein were assessed by Western blot .Results Compared with NS group ,serum amylase ,ALT and AST levels at each time point significantly increased in AP group(P< 0 .01);serum amylase ,ALT and AST levels in AP‐ROS group were significantly lower than those in AP group(P<0 .01) .Compared with NS group ,the expressions of liver PPARγ mRNA and protein in AP group were markedly lower at 6 h and 12 h(P<0 .05) ,and the expressions of PPARγmRNA and protein in AP‐ROS group were significantly higher than those in NS group and AP group(P<0 .01) .The expressions of liver NF‐κB mRNA and NF‐κB p65 protein in AP group were significantly higher than those in NS group and AP‐ROS group at all time points(P<0 .01) .Con‐clusion There are clear relationships between NF‐κB and hepatic injury in acute pancreatitis .The expressions of PPARγin injuried hepatic decreased .Rosiglitazone can increase the expressions of PPARγand prevent the expressions of NF‐κB in hepatic during the early phase of acute pancreatitis .
ABSTRACT
Sixty five patients with of ankylosing spondylitis were randomly divided into intervention group (n =32) and control group (n =33).Patients in control group received conventional treatment only ;in addition to conventional treatment,patients in intervention group also received community intervention,including health education,psychological counseling and joint function exercise.The treatment lasted for 6 months,29 patients in intervention group and 28 in control group completed the trial.The results showed that the spinal motion (flexion,extension,lateral bending),chest expantion,finger to floor distance,occiput to wall gap in intervention group were better improved than those in control group after treatment (P ＜ 0.05).The scores of self-rating anxiety scale (SAS) and self-rating depression scale (SDS) were lower in intervention group than those in control group after treatment (P ＜ 0.05).The results indicate that community intervention can effectively improve spinal mobility,reduce spinal deformity; relieve the anxiety and depression and improve the quality of life for patients with ankylosing spondylitis.